Residual HEK293 DNA Quantitation Kit (3G)

For Research Use Only (RUO)

Product Number: 1101104-1

SHENTEK® human embryonic kidney 293 Kit is used to quantitate residual human embryonic kidney 293 cells DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the HEK293 Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® human embryonic kidney cells DNA Sample Preparation Kit User Guide (Product No. 1104191).

Overview - SHENTEK® Residual HEK293 DNA Quantitation Kit (3G)

Components Shipping Condition Unit Size Detection Method

HEK293 DNA Control -20℃ 100 Reactions Probe-based qPCR

qPCR Master MIX -20℃, protect from light

DNA Dilution Buffer (DDB) -20℃

HEK293 Primer&Probe MIX (Incl IPC) -20℃, protect from light

Specification - SHENTEK® Residual HEK293 DNA Quantitation Kit (3G)

Range 3x10-2-3x102 pg/μL, R2=0.99984, E=98.5%

Accuracy Recovery = 82.1%-104.0%, CV<30%

LOQ 0.03 pg/μL

Precision 8.9%-15.8% (<30%)

Robustness Freeze-thaw stability over 5 cycles

Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipment

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